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ATCC hct15
Hct15, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1650 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hct+15/HCT-15/pm42319820-263-14-21
Average 97 stars, based on 1650 article reviews

hct15 - by Bioz Stars, 2026-09

97/100 stars

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Cell Culture:

Article Title: Proteasome inhibitor, ixazomib prevents topoisomerase-I degradation and reverses irinotecan resistance in colorectal cancer.
Article Snippet: .. Cell culture and drug treatment Human colorectal cancer cell lines DLD-1 (RRID: CVCL_0248; JCRB9090), HCT-15 (RRID: CVCL_ 0292; ATCC CCL-225), and HCT-116 (RRID: CVCL_0291; ATCC CCL-247) were obtained from the Japanese Cancer Research Resources Bank (JCRB, Tokyo, Japan) and the American Type Culture Collection (ATCC, Manassas, VA, USA), respectively. .. Cells were cultured in RPMI-1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U mL 1 penicillin, and 100 lg mL 1 streptomycin.

Article Title: A novel microprotein MUCP1 promotes colorectal cancer metabolic reprogramming by regulating mitochondrial succinate transport
Article Snippet: .. Human colorectal cells HCT 8, HCT 15, HCT 116, SW620, SW480, Lovo, FHC, human liver cells Hep G2 and Hep3b, human breast cells MCF10A, MB-231, MCF7, and T-47D, along with HEK293T cells, were procured from the ATCC (USA) or Chinese academy of science Cell bank (Shanghai, China) and cultured under standard conditions. ..

Multiple Displacement Amplification:

Article Title: Redox cycling nitroxide limits cellular iron availability and selectively inhibits iron-sulfur cluster metabolism
Article Snippet: PPAT (15401-1-AP), LIAS (11577-1-AP) were from Proteintech. .. Cells: 5637, A-172, 786-O, A-253, A-375, A549, ACHN, AGS, AN3 CA, AsPC-1, BT-20, BT-549, BxPC-3, CAL 27, Calu-1, CAMA-1, DBTRG-05MG, Detroit 562, DoTc2 4510, DU 145, FaDu, HCC70, HCC827, HCT-15, HEC-1-A, HEC-1-B, Hep G2, Hs 578T, HT-1080, J82, LN-229, LNCaP, MDA-MB-231, MIA PaCa-2, NCI-H2052, NCI-H647, NCI-N87, PC-3, SH-SY5Y, SK-ES-1, SK-MEL-2, SK-OV-3, SNU-387, SNU-423, TT, U-2 OS, U-87 MG, UM-UC-3 from American Type Culture Collection; 647-V, BEN, CAL-33, EFM19, KYSE-30, OE-33, SK-MEL-30 from DSMZ, KP-2, OUMS-23 from JCRB, Chemicals: RPMI (MT10040CV) was from Corning; PrimeStar HS DNA Polymerase Premix (R040A) was from Takara; RNeasy Plus Mini Kit (74136), Qiaprep Spin Miniprep Kit (27106), and QIAquick Gel Extraction Kit (28706) were from Qiagen. .. 4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy (Tempol, 27051), Erastin (S7242) was from Selleckchem. (+)-Sodium L-ascorbate(A4034), 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox, 238813), Pyridoxal isonicotinoyl hydrazine (PIH, 528110), deferoxamine mesylate (D9533), ferrostatin-1 (SML0583), iron(III) nitrate (F8508), Hydrogen Peroxide(216763) were from Sigma-Aldrich; KI696 (HY-101140) from MedChemExpress shRNAs were obtained from the The RNAi Consortium (TRC) or identical sequences cloned into pLKO.1p: shGFP, TRCN0000072186; shNFS1, TRCN0000229753.

Article Title: Redox cycling nitroxide limits cellular iron availability and selectively inhibits iron-sulfur cluster metabolism.
Article Snippet: PPAT (15401-1-AP), LIAS (11577-1-AP) were from Proteintech. .. Cells: 5637, A-172, 786-O, A-253, A-375, A549, ACHN, AGS, AN3 CA, AsPC-1, BT-20, BT-549, BxPC-3, CAL 27, Calu-1, CAMA-1, DBTRG-05MG, Detroit 562, DoTc2 4510, DU 145, FaDu, HCC70, HCC827, HCT-15, HEC-1-A, HEC-1-B, Hep G2, Hs 578T, HT-1080, J82, LN-229, LNCaP, MDA-MB-231, MIA PaCa-2, NCI-H2052, NCI-H647, NCI-N87, PC-3, SH-SY5Y, SK-ES-1, SK-MEL-2, SK-OV-3, SNU-387, SNU-423, TT, U-2 OS, U-87 MG, UM-UC-3 from American Type Culture Collection; 647-V, BEN, CAL-33, EFM19, KYSE-30, OE-33, SK-MEL-30 from DSMZ, KP-2, OUMS-23 from JCRB, Chemicals: RPMI (MT10040CV) was from Corning; PrimeStar HS DNA Polymerase Premix (R040A) was from Takara; RNeasy Plus Mini Kit (74136), Qiaprep Spin Miniprep Kit (27106), and QIAquick Gel Extraction Kit (28706) were from Qiagen. .. 4-hydroxy-2,2,6,6tetramethyl-1-piperidinyloxy (Tempol, 27051), Erastin (S7242) was from Selleckchem. (+)-Sodium L-ascorbate(A4034), 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox, 238813), Pyridoxal isonicotinoyl hydrazine (PIH, 528110), deferoxamine mesylate (D9533), ferrostatin-1 (SML0583), iron(III) nitrate (F8508), Hydrogen Peroxide(216763) were from Sigma-Aldrich; KI696 (HY-101140) from MedChemExpress shRNAs were obtained from the The RNAi Consortium (TRC) or identical sequences cloned into pLKO.1p: shGFP, TRCN0000072186; shNFS1, TRCN0000229753.

Gel Extraction:

Image Search Results

In vitro cytotoxicity of CIK cells against HCT-15 CRC cells at various E:T ratios. The percentage of viable tumor cells was normalized to their respective control. Data are presented as mean ± SD from technical triplicates. Statistical analysis was assessed using one-way ANOVA. **** p < 0.0001.

Journal: ImmunoTargets and Therapy

Article Title: An Exploratory in vitro Study of Cytotoxicity and Cytokine Profiling of Cytokine-Induced Killer (CIK) Cells Against HCT-15 Colorectal Cancer (CRC) Cells

doi: 10.2147/ITT.S594441

Figure Lengend Snippet: In vitro cytotoxicity of CIK cells against HCT-15 CRC cells at various E:T ratios. The percentage of viable tumor cells was normalized to their respective control. Data are presented as mean ± SD from technical triplicates. Statistical analysis was assessed using one-way ANOVA. **** p < 0.0001.

Article Snippet: The human colorectal cancer (CRC) cell line HCT-15 was obtained from the American Type Culture Collection (ATCC; USA).

Techniques: In Vitro, Control

Cytotoxic effects of 5-FU and CIK cells on HCT-15 cells. HCT-15 were pre-treated with 5-FU (0.050, 0.075, and 0.100 mg/mL) for 24 hours, followed by co-culture with CIK cells for 72 hours at E:T ratios of 1:5, 1:1, 10:1, and 20:1. ( A ) Cytotoxic effect of 5-FU on HCT-15 cells. ( B ) Cytotoxic effect of CIK cells at various E:T ratios on HCT-15 cells. ( C ) Combined effects of 5-FU and CIK cells on HCT-15 cells. Statistical analyses for ( A and B ) were performed using one-way ANOVA, while ( C ) was analyzed using two-way ANOVA. Data are presented as mean ± SD from technical triplicates. * p < 0.05, *** p < 0.001, **** p < 0.0001, compared with the control group.

Journal: ImmunoTargets and Therapy

Article Title: An Exploratory in vitro Study of Cytotoxicity and Cytokine Profiling of Cytokine-Induced Killer (CIK) Cells Against HCT-15 Colorectal Cancer (CRC) Cells

doi: 10.2147/ITT.S594441

Figure Lengend Snippet: Cytotoxic effects of 5-FU and CIK cells on HCT-15 cells. HCT-15 were pre-treated with 5-FU (0.050, 0.075, and 0.100 mg/mL) for 24 hours, followed by co-culture with CIK cells for 72 hours at E:T ratios of 1:5, 1:1, 10:1, and 20:1. ( A ) Cytotoxic effect of 5-FU on HCT-15 cells. ( B ) Cytotoxic effect of CIK cells at various E:T ratios on HCT-15 cells. ( C ) Combined effects of 5-FU and CIK cells on HCT-15 cells. Statistical analyses for ( A and B ) were performed using one-way ANOVA, while ( C ) was analyzed using two-way ANOVA. Data are presented as mean ± SD from technical triplicates. * p < 0.05, *** p < 0.001, **** p < 0.0001, compared with the control group.

Article Snippet: The human colorectal cancer (CRC) cell line HCT-15 was obtained from the American Type Culture Collection (ATCC; USA).

Techniques: Co-Culture Assay, Control

In vitro cytotoxic activity of CIK cells against HCT-15 cells. The cytotoxic effect of CIK cells expanded from healthy donor 3 versus CRC patient was assessed at E:T ratios of 1:5, 1:2, 1:1, 5:1, 10:1, 20:1 and 40:1. Cancer cells without CIK cells served as normalizing controls for both healthy donor and cancer patient. Statistical analysis was assessed using independent t -test. Data are presented as mean ± SD from technical triplicates. ** p < 0.01, **** p < 0.0001, compared to the respective control.

Journal: ImmunoTargets and Therapy

Article Title: An Exploratory in vitro Study of Cytotoxicity and Cytokine Profiling of Cytokine-Induced Killer (CIK) Cells Against HCT-15 Colorectal Cancer (CRC) Cells

doi: 10.2147/ITT.S594441

Figure Lengend Snippet: In vitro cytotoxic activity of CIK cells against HCT-15 cells. The cytotoxic effect of CIK cells expanded from healthy donor 3 versus CRC patient was assessed at E:T ratios of 1:5, 1:2, 1:1, 5:1, 10:1, 20:1 and 40:1. Cancer cells without CIK cells served as normalizing controls for both healthy donor and cancer patient. Statistical analysis was assessed using independent t -test. Data are presented as mean ± SD from technical triplicates. ** p < 0.01, **** p < 0.0001, compared to the respective control.

Article Snippet: The human colorectal cancer (CRC) cell line HCT-15 was obtained from the American Type Culture Collection (ATCC; USA).

Techniques: In Vitro, Activity Assay, Control

The relative levels of cytokines in the conditioned media of ( A ) CIK cells from donor 3 and ( B ) HCT-15 cells were visualized using a human cytokine antibody array (Proteome Profiler Human XL Cytokine Array Kit, R&D Systems). The following highly expressed markers in CIK cells and HCT-15 cells culture are indicated by the boxes on the membranes: A: Adiponectin; B: Apolipoprotein A-I; C: Angiogenin; D: Complement component C5/C5a; E: Fas ligand (FasL); F: Granulocyte macrophage colony-stimulating factor (GM-CSF); G: Interleukin-2 (IL-2); H: Interleukin-5 (IL-5); I: Interleukin-8 (IL-8); J: Macrophage inflammatory protein-1 alpha/Macrophage inflammatory protein-1 beta (MIP-1α/MIP-1β); K: Platelet factor 4 (PF4); L: Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES); M: Retinol binding protein 4 (RBP-4); N: Cluster of differentiation 31 (CD31); O: T-cell immunoglobulin and mucin domain 3 (TIM-3); P: Dickkopf-related protein 1 (DKK-1); Q: Growth differentiation factor 15 (GDF-15); R: Human interferon-inducible protein 10 (IP-10); S: Lipocalin-2; T: Monokine induced by gamma interferon (MIG); U: Serpin E1; V: Vascular endothelial growth factor (VEGF). Differences in spot intensity reflect relative cytokine expression levels, where darker spots indicate higher expression and lighter spots indicate lower expression. Background signal was subtracted during image analysis.

Journal: ImmunoTargets and Therapy

Article Title: An Exploratory in vitro Study of Cytotoxicity and Cytokine Profiling of Cytokine-Induced Killer (CIK) Cells Against HCT-15 Colorectal Cancer (CRC) Cells

doi: 10.2147/ITT.S594441

Figure Lengend Snippet: The relative levels of cytokines in the conditioned media of ( A ) CIK cells from donor 3 and ( B ) HCT-15 cells were visualized using a human cytokine antibody array (Proteome Profiler Human XL Cytokine Array Kit, R&D Systems). The following highly expressed markers in CIK cells and HCT-15 cells culture are indicated by the boxes on the membranes: A: Adiponectin; B: Apolipoprotein A-I; C: Angiogenin; D: Complement component C5/C5a; E: Fas ligand (FasL); F: Granulocyte macrophage colony-stimulating factor (GM-CSF); G: Interleukin-2 (IL-2); H: Interleukin-5 (IL-5); I: Interleukin-8 (IL-8); J: Macrophage inflammatory protein-1 alpha/Macrophage inflammatory protein-1 beta (MIP-1α/MIP-1β); K: Platelet factor 4 (PF4); L: Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES); M: Retinol binding protein 4 (RBP-4); N: Cluster of differentiation 31 (CD31); O: T-cell immunoglobulin and mucin domain 3 (TIM-3); P: Dickkopf-related protein 1 (DKK-1); Q: Growth differentiation factor 15 (GDF-15); R: Human interferon-inducible protein 10 (IP-10); S: Lipocalin-2; T: Monokine induced by gamma interferon (MIG); U: Serpin E1; V: Vascular endothelial growth factor (VEGF). Differences in spot intensity reflect relative cytokine expression levels, where darker spots indicate higher expression and lighter spots indicate lower expression. Background signal was subtracted during image analysis.

Article Snippet: The human colorectal cancer (CRC) cell line HCT-15 was obtained from the American Type Culture Collection (ATCC; USA).

Techniques: Ab Array, Activation Assay, Binding Assay, Expressing

Differentially expressed cytokines and growth factors in the conditioned medium of CIK cells from donor 3 alone, HCT-15 cells, and co-culture of CIK cells and HCT-15 cells at E:T ratios of 1:1 and 10:1. ( A ) Heatmap of 105 cytokines and chemokines. ( B ) Venn diagram depicting markers that were highly expressed (pixel density >300,000) in CIK cells and HCT-15 cells.

Journal: ImmunoTargets and Therapy

Article Title: An Exploratory in vitro Study of Cytotoxicity and Cytokine Profiling of Cytokine-Induced Killer (CIK) Cells Against HCT-15 Colorectal Cancer (CRC) Cells

doi: 10.2147/ITT.S594441

Figure Lengend Snippet: Differentially expressed cytokines and growth factors in the conditioned medium of CIK cells from donor 3 alone, HCT-15 cells, and co-culture of CIK cells and HCT-15 cells at E:T ratios of 1:1 and 10:1. ( A ) Heatmap of 105 cytokines and chemokines. ( B ) Venn diagram depicting markers that were highly expressed (pixel density >300,000) in CIK cells and HCT-15 cells.

Article Snippet: The human colorectal cancer (CRC) cell line HCT-15 was obtained from the American Type Culture Collection (ATCC; USA).

Techniques: Co-Culture Assay

Distinct protein-protein interaction among the significantly regulated cytokines and growth factors within ( A and B ) CIK cells from donor 3 or ( C and D ) HCT-15 cells during co-culture, as elucidated by STRING. ( A ) Functional and physical associations of cytokines significantly regulated within CIK cells. ( B ) Association strengths of these regulated factors in different biological processes. Red: positive regulation of mononuclear cell proliferation; Green: positive regulation of immune system process; Blue: leukocyte migration, Yellow: negative regulation of cellular process; Pink: positive regulation of cellular process. ( C ) Functional and physical associations of cytokines significantly regulated within HCT-15 cells. ( D ) Association strengths of these regulated factors in different biological processes. Yellow: positive regulation of immune system process; Green: positive regulation of the MAPK cascade; Red: regulation of cell population proliferation; Pink: regulation of cell communication. The networks were generated using the STRING database. Nodes represent proteins, and edges indicate predicted or known associations. These interactions reflect database-derived relationships and suggest potential functional connectivity but do not imply direct causal interactions.

Journal: ImmunoTargets and Therapy

Article Title: An Exploratory in vitro Study of Cytotoxicity and Cytokine Profiling of Cytokine-Induced Killer (CIK) Cells Against HCT-15 Colorectal Cancer (CRC) Cells

doi: 10.2147/ITT.S594441

Figure Lengend Snippet: Distinct protein-protein interaction among the significantly regulated cytokines and growth factors within ( A and B ) CIK cells from donor 3 or ( C and D ) HCT-15 cells during co-culture, as elucidated by STRING. ( A ) Functional and physical associations of cytokines significantly regulated within CIK cells. ( B ) Association strengths of these regulated factors in different biological processes. Red: positive regulation of mononuclear cell proliferation; Green: positive regulation of immune system process; Blue: leukocyte migration, Yellow: negative regulation of cellular process; Pink: positive regulation of cellular process. ( C ) Functional and physical associations of cytokines significantly regulated within HCT-15 cells. ( D ) Association strengths of these regulated factors in different biological processes. Yellow: positive regulation of immune system process; Green: positive regulation of the MAPK cascade; Red: regulation of cell population proliferation; Pink: regulation of cell communication. The networks were generated using the STRING database. Nodes represent proteins, and edges indicate predicted or known associations. These interactions reflect database-derived relationships and suggest potential functional connectivity but do not imply direct causal interactions.

Article Snippet: The human colorectal cancer (CRC) cell line HCT-15 was obtained from the American Type Culture Collection (ATCC; USA).

Techniques: Co-Culture Assay, Functional Assay, Migration, Generated, Derivative Assay

Levels of cytokines and growth factors in HCT-15 cells alone and co-cultured with CIK cells from donor 3 at E:T ratios of 1:1 and 10:1. The factors are grouped based on their functions into ( A ) antitumor, ( B ) immunosuppression, and ( C ) cell proliferation and metastasis. The pixel intensity is shown as mean ± SD from technical triplicates. Two-way ANOVA followed by post-hoc Tukey analysis was performed. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 compared to HCT-15 cells; ** p < 0.01, **** p < 0.0001 compared to CIK:HCT-15 at an E:T ratio of 1:1.

Journal: ImmunoTargets and Therapy

Article Title: An Exploratory in vitro Study of Cytotoxicity and Cytokine Profiling of Cytokine-Induced Killer (CIK) Cells Against HCT-15 Colorectal Cancer (CRC) Cells

doi: 10.2147/ITT.S594441

Figure Lengend Snippet: Levels of cytokines and growth factors in HCT-15 cells alone and co-cultured with CIK cells from donor 3 at E:T ratios of 1:1 and 10:1. The factors are grouped based on their functions into ( A ) antitumor, ( B ) immunosuppression, and ( C ) cell proliferation and metastasis. The pixel intensity is shown as mean ± SD from technical triplicates. Two-way ANOVA followed by post-hoc Tukey analysis was performed. # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 compared to HCT-15 cells; ** p < 0.01, **** p < 0.0001 compared to CIK:HCT-15 at an E:T ratio of 1:1.

Article Snippet: The human colorectal cancer (CRC) cell line HCT-15 was obtained from the American Type Culture Collection (ATCC; USA).

Techniques: Cell Culture

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